As a graduate student, I studied how the decision between a latent and lytic state in Human Immunodeficiency Virus-1 (HIV-1) is regulated by viral gene expression noise. Using quantitative single-cell techniques, I studied how the chromatin environment regulates gene expression dynamics in the mammalian genome.
Since traditional single-cell methods, such as single-molecule mRNA FISH, allow quantification of only a handful of parameters concurrently, as a post-doc I started developing new genome-wide techniques that enables sequencing both genomic DNA and mRNA from the same single cell. I am currently working towards automating such integrated single-cell methods and extending such genome-wide techniques to epigenetic measurements in single cells.
Gene expression noise, single-cell genomics, single-cell epigenomics, transcription, epigenetics, quantitative biology and dynamical systems